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- visitors In vivo analysis of intracellular amino acid labelings by GC/MS. C Wittmann, M Hans, E Heinzle - Analytical biochemistry, 2002 - mendeley.com ... TBDMS derivatization of amino acids is especially useful in metabolic network analysis, because M-57 fragments containing the entire carbon skeleton of the analyte can be observed by GC/MS with high signal intensities 8. The potential of the method is exemplified for ... Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose nih.gov [HTML]P Kiefer, E Heinzle, O Zelder, C … - Applied and …, 2004 - Am Soc Microbiol ... A comprehensive approach to 13 C tracer studies, labeling measurements by gas chromatography-mass spectrometry, metabolite balancing, and isotopomer modeling, was applied for comparative metabolic network analysis of lysine-producing Corynebacterium glutamicum ... It is all about metabolic fluxes nih.gov [HTML]J Nielsen - Journal of bacteriology, 2003 - Am Soc Microbiol http //scholar.google.com/scholar?q=related xAvLLrdbbNYJ scholar.google.com/ hl=ja as_sdt=2000 ... I. Experimental observations. Biotechnol. Bioeng. 55 305-316.[CrossRef] 21; Thykaer, J., B. Christensen, and J. Nielsen. 2002. Metabolic network analysis of an adipoyl-7-ADCA producing strain of Penicillium chrysogenum elucidation of adipate degradation.Metab. Eng. ... Using topology of the metabolic network to predict viability of mutant strains biomedcentral.com [PDF]Z Wunderlich, L Mirny - Genome Biology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related sTavonuje7wJ scholar.google.com/ hl=ja as_sdt=2000 ... Ph.D. Thesis. University of Leeds; 1994. 14. Papp B, Pal C, Hurst LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 2004, 429(6992) 661-664. 15. Jeong H, Tombor B, Albert R, Oltvai ZN, Barabasi AL The large-scale ... Novel proteins, putative membrane transporters, and an integrated metabolic network are revealed by quantitative proteomic analysis of Arabidopsis cell … plantphysiol.org [HTML]H Eubel, EH Meyer, NL Taylor, JD Bussell, N O … - Plant …, 2008 - Am Soc Plant Biol http //scholar.google.com/scholar?q=related aCgbR-dAtyMJ scholar.google.com/ hl=ja as_sdt=2000 ... This is aided by a metabolic network analysis that reveals a tight integration of functions and highlights specific metabolite nodes that most probably represent entry and exit metabolites that could require transport across the peroxisomal membrane. ... High-throughput phenomics experimental methods for mapping fluxomes bjmu.cn [PDF]U Sauer - Current opinion in Biotechnology, 2004 - Elsevier http //scholar.google.com/scholar?q=related XvhSxXXwbn4J scholar.google.com/ hl=ja as_sdt=2000 ... From its early days when material fluxes were balanced within assumed reaction networks [7.], metabolic network analysis [6. and 8.] has matured to actually identify the topology of active reactions and pathways and to quantify the molecular flux through them on a variety of ... Use of genome-scale microbial models for metabolic engineering KR Patil, M Åkesson, J Nielsen - Current opinion in biotechnology, 2004 - Elsevier http //scholar.google.com/scholar?q=related oAOz5SBTVHAJ scholar.google.com/ hl=ja as_sdt=2000 ... incorporate genome-scale biological data. Genome-scale stoichiometric models of microorganisms represent a first step in this direction. FBA, flux balance analysis; MFA, metabolic flux analysis; MNA, metabolic network analysis. ... Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression nih.gov [HTML]AK Gombert, M Moreira dos Santos, B … - Journal of …, 2001 - Am Soc Microbiol http //scholar.google.com/scholar?q=related R5V_AodxCrwJ scholar.google.com/ hl=ja as_sdt=2000 ... flux estimates. The use of 13 C-labeled substrates enables both identification of the metabolic network structure and quantification of the metabolic fluxes and is therefore referred to as metabolic network analysis (5). This kind ... Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method freelogy.org [PDF]J Zhao, K Shimizu - Journal of biotechnology, 2003 - Elsevier http //scholar.google.com/scholar?q=related w1m-5IKYKpsJ scholar.google.com/ hl=ja as_sdt=2000 Structural and functional analysis of cellular networks with CellNetAnalyzer biomedcentral.com [HTML]S Klamt, J Saez-Rodriguez, ED Gilles - BMC Systems Biology, 2007 - biomedcentral.com http //scholar.google.com/scholar?q=related bzwlVku0FL8J scholar.google.com/ hl=ja as_sdt=2000 ... CNA extends its predecessor FluxAnalyzer, originally developed for metabolic network analysis[7], by new methods for signalling and regulatory networks, ie for networks where signal flows are dominating (in contrast to mass flows in metabolic networks). ...
https://w.atwiki.jp/yoshida2/pages/115.html
telnetでブラウズ(HTTP) このページでは、インターネットでホームページなどをブラウジングするときに利用するHTTPプロトコルについて説明しています。 概要 HTTPプロトコルとは、Hypertext Transfer Protocolの略で、インターネットでホームページなどをブラウジングするときに利用しているプロトコルです。 HTTPプロトコルは、TCP/IP上のプロトコルで、通常80番ポートを使ってアクセスします。 詳細な定義は、以下のRFCで定義されています。 RFC-1945 HTTP/1.0 RFC-2068 HTTP/1.1 基本的に、メッセージを要求(リクエスト)し、その応答結果(レスポンス)を表示するだけです。 HTTPのアクセスログ ApacheなどのWebサーバのログを見ると、リクエストとレスポンスが、以下の形式で出力されます。 アクセスログの書式 アクセス元 - - [アクセス時間] メソッド名 URL名 プロトコルバージョン レスポンスコード 例： 10.0.1.20 - - [01/Jul/1999 23 22 46 +0900] GET /usr/joe/index.html HTTP/1.0 200 2060 メソッドの種類には、GETやPOSTなどがあります。 GETメソッドは、HTMLデータや画像データなどを取得する場合に使用します。 GETメソッドで要求すると、ヘッダ情報とHTMLデータが返却されます。 GETメソッドでCGIを呼び出すこともできます。 パラメータを渡す場合は、URLの後に ?name1=データ1 name2=データ2 という形式で指定します。 HEADメソッドは、ファイルサイズや更新日時を調べる場合に利用します。 HEADメソッドを利用すると、ヘッダ情報だけが返却されます。 POSTメソッドは、CGIなどにデータを渡す場合に使用します。 パラメータなどのデータは、環境変数などによって、CGIに渡ります。 以下に、HTTPのバージョンとメソッドの種類を示します。 メソッドの種類 HTTP/1.0 GET, HEAD, PUT, POST, DELETE, LINK, UNLINK HTTP/1.1 OPTIONS, TRACE, PATCH 以下に、レスポンスコードの一覧を示します。 100番台は通信エラー、200番台は正常終了、300番台はリダイレクト、400番台はクライアントエラー、500番台はサーバエラーを表しています。 リダイレクトとは、クライアントの指定したURLに移動した場合などに返却されます。 レスポンスコード コード 内容 コード 内容 100 Continue 101 Switching Protocols 200 OK 201 Created 202 Accepted 203 Non-Authoritative Information 204 No Content 205 Reset Content 206 Partial Content 300 Multiple Choices 301 Moved Permanently 302 Moved Temporarily 303 See Other 304 Not Modified 305 Use Proxy 400 Bad Request 401 Unauthorized 402 Payment Required 403 Forbidden 404 Not Found 405 Method Not Allowed 406 Not Acceptable 407 Proxy Authentication Required 408 Request Time-out 409 Conflict 410 Gone 411 Length Required 412 Precondition Failed 413 Request Entity Too Large 414 Request-URI Too Large 415 Unsupported Media Type 500 Internal Server Error 501 Not Implemented 502 Bad Gateway 503 Service Unavailable 504 Gateway Time-out 505 HTTP Version not supported レスポンスコードは、ログファイルなどに出力されますので、知っていると役に立つと思います。 telnetでブラウジング では、実際にtelnetでブラウジングしてみましょう。 telnetコマンドで、WWWサーバ名と、80番ポートを指定します。 Linux# telnet ash.jp 80 そこで、環境変数をすべて表示するCGI(env.cgi)を起動してみます。 まず、GETコマンドで、取得したいURLと、HTTPのバージョンを指定します。 GET /~joe/prog/cgi/env01.cgi HTTP/1.0 ここで、リターンのみを入力しても表示されますが、以下のオプションも入力してみます。 指定したオプションは、環境変数としてCGIに渡ります。 User-Agent Telnet [ja] (Linux) Host www.ash.jp リターンのみを入力すると、レスポンスと、HTMLが表示されます。 当然、HTMLのソースが表示されるだけですから、頭の中でHTMLを解析し画面イメージを想像する必要があります。 以下に実行結果を示します。 Linux# telnet ash.jp 80 Trying 210.154.87.18... Connected to ash.jp. Escape character is ^] . GET /~joe/prog/cgi/env01.cgi HTTP/1.0 User-Agent Telnet [ja] (Linux) Host ash.jp HTTP/1.1 200 OK DeleGate-Ver 5.8.8 (delay=7) Date Fri, 02 Jul 1999 09 56 24 GMT Server Apache/1.3.3 (Unix) Content-Type text/html Via 1.0 210.154.87.18 80 (DeleGate/5.8.8) Content-Length 954 html body h1 ＣＧＩでの環境変数一覧 /h1 hr h3 環境変数の表示 /h3 DOCUMENT_ROOT = /usr/local/share/apache/htm br GATEWAY_INTERFACE = CGI/1.1 br HTTP_FORWARDED = by http //210.154.87.18 80/ (DeleGate/5.8.8) for sv01.lo.ash.or .jp br HTTP_HOST = ash.jp br HTTP_USER_AGENT = Telnet [ja] (Linux) br HTTP_VIA = 1.0 210.154.87.18 80 (DeleGate/5.8.8) br PATH = /usr/local/sbin /usr/local/bin /sbin /usr/sbin /bin /usr/bin br QUERY_STRING = br REMOTE_ADDR = 10.0.1.254 br REMOTE_PORT = 12621 br REQUEST_METHOD = GET br REQUEST_URI = /~joe/prog/cgi/env01.cgi br SCRIPT_FILENAME = /home/joe/public_html/prog/cgi/env01.cgi br SCRIPT_NAME = /~joe/prog/cgi/env01.cgi br SERVER_ADMIN = info@ash.jp br SERVER_NAME = ash.jp br SERVER_PORT = 80 br SERVER_PROTOCOL = HTTP/1.0 br SERVER_SIGNATURE = ADDRESS Apache/1.3.3 Server at ash.jp Port 80 /ADDRESS br SERVER_SOFTWARE = Apache/1.3.3 (Unix) br hr br このＣＧＩのソース： a href=env01.txt env01.cgi /a br /body /html Connection closed by foreign host. 最初の、HTTP/1.1 200 OKは、HTTPプロトコルで、正常に転送したことを表します。 その後にヘッダ情報が転送され、最後にHTMLのソースが転送されます。 環境変数として、以下の値が追加設定されているのが確認できます。 HTTP_HOST = ash.jp HTTP_USER_AGENT = Telnet [ja] (Linux) このように、HTTPプロトコルは、要求（リクエスト）に対して、応答（レスポンス）が返却されるだけのシンプルなプロトコルです。 余談ですが、HTTPプロトコルという呼び方は、Hyper Text Transfer Protocol プロトコルになって、プロトコルが2重になってしまい、おかしいです。 でも、この方がわかりやすいので、私はよく使います。
https://w.atwiki.jp/katsuhiro/pages/26.html
HTTPメソッド GET情報取得メソッド POST PUT DELETEリソース削除メソッド HTTPリクエスト ポストデータ HTMLのPOST指定のformから送信されるデータ JSPのrequestオブジェクトから下記のメソッドで情報を取得できるgetParameter getParameterValues リクエストボディのセットされて送信される クエリ情報 URLの末尾の"?"以降に「キー＝値」のセットで付加される簡易な情報 複数のセットがある場合は" "で連結される URLに直接指定するほか、HTMLのGET指定のformから送信されるデータ formのmethod属性が省略された場合もクエリ情報として送信される JSPのrequestオブジェクトから下記のメソッドで情報を取得できるgetParameter クエリ情報に特殊文字が含まれる場合は、あらかじめエンコードしておく必要がある（form経由の場合はエンコード不要） URLに使用可能な文字数を超えて送信できない(2,083文字)FireFoxの場合は制限が無いが、動作が遅くなる場合もあるらしい データがアドレス欄に露出してしまう ヘッダ情報 「名前： 値」形式 種類一般ヘッダ（要求／応答時双方で利用） エンティティヘッダ（コンテンツに関する情報） リクエスト（要求）ヘッダ（クライアントに関する情報） レスポンス（応答）ヘッダ（その他） JSPのrequestオブジェクトから下記のメソッドで情報を取得できるgetHeaderNames ただし、情報取得専用メソッドが存在するヘッダについてはそちらを使う ■ヘッダ例 種類 ヘッダ名 概要 一般 Cache-Control キャッシュルールを規定する Connection プロキシ（中間）サーバで削除すべきHTTPヘッダを指定 Date コンテンツ生成日時 Pragma キャッシングを利用するか（下位互換用のヘッダ） Transfer-Encoding コンテンツの転送エンコーディング方式 リクエスト Accept クライアント対応コンテンツの種類（優先順） Accept-Language クライアント対応言語（優先順） Authorization 認証情報 Cookie クライアントに保存されたクッキーデータを送信 Host 要求先ホスト名 If-Modified-Since 指定日時以降にコンテンツが更新されている場合にのみ、サーバはデータを送信 Proxy-Authorization プロキシサーバ用の認証情報 Range 要求リソース範囲 Referer リンク元URI User-Agent クライアントの種類 レスポンス ETag リソースを一意に特定するためのキー情報（コンテンツが更新されていないかどうかを特定する場合などに使用） Location クライアントに新しいURIに移動するように促す Server サーバの種類 Set-Cookie クライアントにクッキーを送信 WWW-Authenticate クライアントに認証を要求 エンティティ Content-Encoding コンテンツのエンコーディング方式 Content-Length コンテンツサイズ Content-Type コンテンツの種類 Expires コンテンツの有効期限 Last-Modified コンテンツの最終更新年月日 クッキー サーバがクライアントに対して自由に読み書きできるテキスト クッキーの有効期限負数をセットした場合、ブラウザの終了時に削除される ０をセットした場合、その場で削除される １以上をセットすると、その秒数分保持される クッキーのデメリットクライアント側でクッキーを受け入れないように設定することが可能 クライアント側でクッキーの改竄や削除が可能 実データがネットワーク上を流れてしまう クッキーの制約1つのホスト、ドメイン当たりの最大個数は20個（総数では300個以内） クッキー1つあたりの最大サイズは4096バイト セッション情報 長期間にわたって情報を保持するには不向き ユーザがブラウザを開いている間だけアプリケーション内で情報を受け渡しする場合にはクッキーより手軽でセキュアな仕組み sessionJSPの暗黙オブジェクトの１つ 下記のメソッドを使って読み書きするgetAttribute setAttribute @pageディレクティブのsession属性がfalseの場合は使えない sessionが不要の場合はsession属性をfalseにしておく（サーバリソースの節約） sessionを使うと… クライアントからサーバにリクエスト送信 サーバからのレスポンスにSet-Cookieヘッダの値としてセッションIDが付加される(JSESSIONID) クライアントからサーバに2回目以降のリクエスト送信時、Cookieヘッダの値として、受け取ったJSESSIONIDを付加して送る サーバをそのセッションIDに該当するセッションを使って処理をし、レスポンスを返す セッションの破棄方法明示的な破棄(session.invalidate()) 有効期限を設定する(session.setMaxInactiveInteval()) web.xmlにセッションの有効期限を設定する session-config session-timeout セッションの寿命明示的に破棄されるか有効期限を過ぎない限り、クライアントを閉じてもサーバ上で生き続ける ieHTTPHeaders http //www.blunck.info/iehttpheaders/iehttpheaders.html ⇒IE上でHTTPによる通信内容をトレースするツール
https://w.atwiki.jp/metalmetabo/pages/29.html
- visitors Genome-scale reconstruction of the metabolic network in Staphylococcus aureus N 315 an initial draft to the two-dimensional annotation biomedcentral.com [HTML]SA Becker, BØ Palsson - BMC microbiology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related 829ZIciJNnkJ scholar.google.com/ hl=ja as_sdt=2000 ... J Biol Chem 2004, 279 39532-39540. PubMed Abstract | Publisher Full Text OpenURL. Papp B, Pal C, Hurst LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 2004, 429 661-664. ... Adaptive evolution of bacterial metabolic networks by horizontal gene transfer linkgroup.hu [PDF]C Pál, B Papp, MJ Lercher - Nature Genetics, 2005 - nature.com http //scholar.google.com/scholar?q=related j65eRY_pFyIJ scholar.google.com/ hl=ja as_sdt=2000 ... were present. Gene families with more than one member in E. coli K-12 were excluded from the analysis. Metabolic network analysis. We examined the reconstructed metabolic network (iJR904 GSM/GPR) of E. coli K-12. We ... Network analysis of metabolic enzyme evolution in Escherichia coli biomedcentral.com [HTML]S Light, P Kraulis - BMC bioinformatics, 2004 - biomedcentral.com http //scholar.google.com/scholar?q=related M0gfPqO-WsQJ scholar.google.com/ hl=ja as_sdt=2000 ... representation of metabolic pathways where the substrates and products are the vertices and the enzymes catalyzing the reactions are the edges (Figure 1). The type of network representation used in our study has been used before for metabolic network analysis where it has ... Metabolic network analysis on Phaffia rhodozyma yeast using 13C-labeled glucose and gas chromatography-mass spectrometry C Cannizzaro, B Christensen, J Nielsen, U … - Metabolic Engineering, 2004 - Elsevier http //scholar.google.com/scholar?q=related xVq5KkaMAd4J scholar.google.com/ hl=ja as_sdt=2000 Amino acid labeling patterns, as determined by GC–MS, were in accordance with a metabolic network consisting of the Embden–Meyerhof–Parnas pathway, the pentose phosphate pathway, and the TCA cycle. Glucose was mainly consumed along the pentose ... Metabolic network analysis integrated with transcript verification for sequenced genomes A Manichaikul, L Ghamsari, EFY Hom, C Lin, RR … - Nature methods, 2009 - nature.com http //scholar.google.com/scholar?q=related qlItzvkCLowJ scholar.google.com/ hl=ja as_sdt=2000 Present availability of genome sequences for diverse microorganisms brings opportunities for metabolic engineering through systems-level characterization of these organisms metabolic networks 1 . Such efforts require both functional and structural annotation of metabolic ... High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints E Fischer, N Zamboni, U Sauer - Analytical biochemistry, 2004 - Elsevier http //scholar.google.com/scholar?q=related AGqwTbFzQbYJ scholar.google.com/ hl=ja as_sdt=2000 Influence of metabolic network structure and function on enzyme evolution biomedcentral.com [HTML]D Vitkup, P Kharchenko, A Wagner - Genome biology, 2006 - biomedcentral.com http //scholar.google.com/scholar?q=related tyk--z8xdr0J scholar.google.com/ hl=ja as_sdt=2000 ... Cold Spring Harbor Press, NY; 1982. OpenURL. Papp B, Pal C, Hurst LD Metabolic network analysis of the causes and evolution of the enzyme dispensability in yeast. Nature 2004,429 661-664. PubMed Abstract | Publisher Full Text OpenURL. ... Metabolomics-and proteomics-assisted genome annotation and analysis of the draft metabolic network of Chlamydomonas reinhardtii genetics.org [HTML]P May, S Wienkoop, S Kempa, B Usadel, N … - Genetics, 2008 - Genetics Soc America http //scholar.google.com/scholar?q=related lrqK6GwpxFIJ scholar.google.com/ hl=ja as_sdt=2000 ... Furthermore, the results will contribute to an improvement of metabolic databases. Presently, our methods are limited to one selected large-scale metabolic network analysis technique, the method of network expansion. Further ... Metabolic network analysis during fed-batch cultivation of Corynebacterium glutamicum for pantothenic acid production first quantitative data and analysis of by- … C Chassagnole, A Diano, F Létisse, ND Lindley - Journal of biotechnology, 2003 - Elsevier http //scholar.google.com/scholar?q=related CJC7H27nMvMJ scholar.google.com/ hl=ja as_sdt=2000 A first generation genetically modified strain of Corynebacterium glutamicum has been assessed for its potential to synthesise and accumulate the vitamin pantothenic acid in the medium using fed-batch cultivation technology, with biomass concentration controlled by isoleucine ... Metabolic modeling of microbial strains in silico ru.nl [PDF]MW Covert, CH Schilling, I Famili, JS Edwards, … - Trends in Biochemical …, 2001 - Elsevier http //scholar.google.com/scholar?q=related pjh6sWp3MRMJ scholar.google.com/ hl=ja as_sdt=2000 ... labeling experiments. Adv. Biochem. Eng. Biotechnol. 54, pp. 109–154. Christensen, B. and Nielsen, J., Metabolic network analysis. A powerful tool in metabolic engineering. Adv. Biochem. Eng. Biotechnol. 66, pp. 209–231. The ...
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- visitors Application of regulatory sequence analysis and metabolic network analysis to the interpretation of gene expression data psu.edu [PDF]J Van Helden, D Gilbert, L Wernisch, M Schroeder, … - Computational …, 2001 - Springer http //scholar.google.com/scholar?q=related JS_4j3CP5nEJ scholar.google.com/ hl=ja as_sdt=2000 Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains T Grotkjær, P Christakopoulos, J Nielsen, L … - Metabolic Engineering, 2005 - Elsevier http //scholar.google.com/scholar?q=related m6nOh8HQbaIJ scholar.google.com/ hl=ja as_sdt=2000 The recombinant xylose fermenting strain Saccharomyces cerevisiae TMB3001 can grow on xylose, but the xylose utilisation rate is low. One important reason for the inefficient fermentation of xylose to ethanol is believed to be the imbalance of redox co-factors. In the present ... The Edinburgh human metabolic network reconstruction and its functional analysis nih.gov [HTML]H Ma, A Sorokin, A Mazein, A Selkov, E … - Molecular systems …, 2007 - nature.com http //scholar.google.com/scholar?q=related pgWLepUKeS0J scholar.google.com/ hl=ja as_sdt=2000 ... A main objective of human metabolic network analysis is to see how it is related with human disease. More than 10 000 human genes (half of the whole genome) have been reported to be related with one or more human diseases in the OMIM database (Hamosh et al, 2005). ... Reconstruction of metabolic networks from genome data and analysis of their global structure for various organisms oxfordjournals.org [PDF]H Ma, AP Zeng - Bioinformatics, 2003 - Oxford Univ Press http //scholar.google.com/scholar?q=related SuHZgSLeNw8J scholar.google.com/ hl=ja as_sdt=2000 ... It is one of the main tasks of metabolic network analysis to find possible conversion pathways between two metabolites. There may be many different pathways between two metabolites, among them the shortest pathway is of particular interest for network analysis. ... Metabolic network analysis of an adipoyl-7-ADCA-producing strain of Penicillium chrysogenum elucidation of adipate degradation J Thykaer, B Christensen, J Nielsen - Metabolic Engineering, 2002 - Elsevier http //scholar.google.com/scholar?q=related gycTvJYwT9kJ scholar.google.com/ hl=ja as_sdt=2000 An adipoyl-7-ADCA-producing, recombinant strain of Penicillium chrysogenum was characterized by metabolic network analysis, with special focus on the degradation of adipate and determination of the metabolic fluxes. Degradation of the side-chain precursor, adipate, causes an ... Potential metabolic limitations in lysine production by Corynebacterium glutamicum as revealed by metabolic network analysis JA Hollander - Applied Microbiology and Biotechnology, 1994 - Springer http //scholar.google.com/scholar?q=related BwIeARCnafMJ scholar.google.com/ hl=ja as_sdt=2000 Corynebacterium glutamicum and closely related Brevi- bacterium species are the most important organisms used for the industrial fermentative production of ly- sine. Although wild-type strains do not excrete lysine at all, classical mutation programs have resulted in strains ... Metabolic flux and metabolic network analysis of Penicillium chrysogenum using 2D [13C, 1H] COSY NMR measurements and cumulative bondomer simulation WA van Winden, WM van Gulik, D … - Biotechnology and …, 2003 - Wiley Online Library http //scholar.google.com/scholar?q=related Jy6SBrqyeHAJ scholar.google.com/ hl=ja as_sdt=2000 Abstract At present two alternative methods are avail- able for analyzing the fluxes in a metabolic network (1) combining measurements of net conversion rates with a set of metabolite balances including the cofactor bal- ances, or (2) leaving out the cofactor balances and fitting the ... Metabolic‐flux and network analysis in fourteen hemiascomycetous yeasts LM Blank, F Lehmbeck, U Sauer - FEMS yeast research, 2005 - Wiley Online Library http //scholar.google.com/scholar?q=related ILl2ugZn3ZcJ scholar.google.com/ hl=ja as_sdt=2000 ... (5). Oxaloacetate14 mit was estimated using Eq. (6)[16] (6). 2.6Metabolic-network analysis. We used a previously constructed metabolic network [1,24], which consists of the PP pathway, glycolysis, TCA cycle, gluconeogenesis, malic enzyme and by-product formation reactions. ... 13C metabolic flux analysis W Wiechert - Metabolic Engineering, 2001 - Elsevier http //scholar.google.com/scholar?q=related Eat1pl-yJGQJ scholar.google.com/ hl=ja as_sdt=2000 ... 6. B. Christensen and J. Nielsen, Metabolic network analysis, a powerful tool in metabolic engineering. Adv. Biochem. Eng. ... 209–231. 7. B. Christensen and J. Nielsen, Metabolic network analysis of Penicillium chrysogenum using 13 C-labeled glucose. Biotechnol. Bioeng. ... Multiple knockout analysis of genetic robustness in the yeast metabolic network tau.ac.il [PDF]D Deutscher, I Meilijson, M Kupiec, E Ruppin - Nature genetics, 2006 - nature.com http //scholar.google.com/scholar?q=related X5cH6RUwUF0J scholar.google.com/ hl=ja as_sdt=2000 ... | PubMed |; Kitano, H. Biological robustness. Nat. Rev. Genet. 5, 826–837 (2004). | Article | PubMed | ISI | ChemPort |; Papp, B. , Pál, C. Hurst, LD Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 429, 661–664 (2004). ...
https://w.atwiki.jp/metalmetabo/pages/27.html
- visitors Metabolic network analysis B Christensen, J Nielsen - Bioanalysis and biosensors for bioprocess …, 2000 - Springer http //scholar.google.com/scholar?q=related PiGOMNyMhjYJ scholar.google.com/ hl=ja as_sdt=2000 Metabolic network analysis is a tool for investigating the features that identify the topology of a metabolic network and the relative activities of its individual branches. The pillars of metabolic network analysis are mathematical modeling, allowing for a quantitative analysis, ... Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast B Papp, C Pál, LD Hurst - Nature, 2004 - nature.com http //scholar.google.com/scholar?q=related 0sM60oXJ2jQJ scholar.google.com/ hl=ja as_sdt=2000 Under laboratory conditions 80% of yeast genes seem not to be essential for viability 1 . This raises the question of what the mechanistic basis for dispensability is, and whether it is the result of selection for buffering or an incidental side product. Here we analyse these issues ... Genealogy profiling through strain improvement by using metabolic network analysis metabolic flux genealogy of several generations of lysine-producing … nih.gov [HTML]C Wittmann, E Heinzle - Applied and environmental microbiology, 2002 - Am Soc Microbiol http //scholar.google.com/scholar?q=related 49I4bRT2XYIJ scholar.google.com/ hl=ja as_sdt=2000 A comprehensive approach of metabolite balancing, 13 C tracer studies, gas chromatography-mass spectrometry, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and isotopomer modeling was applied for comparative metabolic ... Metabolic network analysis of Penicillium chrysogenum using 13C-labeled glucose B Christensen, J Nielsen - Biotechnology and …, 2000 - interscience.wiley.com http //scholar.google.com/scholar?q=related 9XjgWNCKNqMJ scholar.google.com/ hl=ja as_sdt=2000 To provide a rational approach for directed genetic alter- ations in microorganisms, often referred to as metabolic engineering (Bailey, 1991; Stephanopoulos, 1999; Yarmush and Berthiaume, 1997), a detailed map of the fluxes in the central metabolism is an essential ... Metabolic network analysis of Bacillus clausii on minimal and semirich medium using 13C-labeled glucose T Christiansen, B Christensen, J Nielsen - Metabolic engineering, 2002 - Elsevier http //scholar.google.com/scholar?q=related GeX9jXLzh3EJ scholar.google.com/ hl=ja as_sdt=2000 Using 13 C-labeled glucose fed to the facultative alkalophilic Bacillus clausii producing the alkaline serine protease Savinase, the intracellular fluxes were quantified in continuous cultivation and in batch cultivation on a minimal medium. The flux through the pentose phosphate ... Genome-scale metabolic network analysis of the opportunistic pathogen Pseudomonas aeruginosa PAO1 nih.gov [HTML]MA Oberhardt, J Puchalka, KE Fryer, VAP … - Journal of …, 2008 - Am Soc Microbiol http //scholar.google.com/scholar?q=related isrhQUVHkSMJ scholar.google.com/ hl=ja as_sdt=2000 Pseudomonas aeruginosa is a major life-threatening opportunistic pathogen that commonly infects immunocompromised patients. This bacterium owes its success as a pathogen largely to its metabolic versatility and flexibility. A thorough understanding of P. aeruginosa s ... A metabolic network stoichiometry analysis of microbial growth and product formation WM Van Gulik, JJ Heijnen - Biotechnology and …, 1995 - interscience.wiley.com http //scholar.google.com/scholar?q=related p9UTGWFLJJUJ scholar.google.com/ hl=ja as_sdt=2000 ... Other work on metabolic network analysis using linear op- timization techniques has been performed on hybridoma cell Esehenchiu coli metabolism,3 .32 and acetate overflow in E. c01i.""~ Following the approach to solve underdetermined metabolic networks by conducting ... Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale C Wittmann, HM Kim, E Heinzle - Biotechnology and …, 2004 - Wiley Online Library http //scholar.google.com/scholar?q=related B7ezo5R5OxIJ scholar.google.com/ hl=ja as_sdt=2000 Abstract We present a straightforward approach com- prising 13C tracer experiments at 200-AL volume in 96-well microtiter plates with on-line measurement of dissolved oxygen for quantitative high-throughput metabolic net- work analysis at a miniaturized scale. This ... Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast biomedcentral.com [HTML]LM Blank, L Kuepfer, U Sauer - Genome Biology, 2005 - biomedcentral.com http //scholar.google.com/scholar?q=related TodhI8VNW54J scholar.google.com/ hl=ja as_sdt=2000
https://w.atwiki.jp/naobe/pages/78.html
ネットワークに戻る RFC2616概要 HTTP/1.1 RFC6265概要 HTTP状態管理機構 パーセントエンコーディング（URLエンコード） URLで意味を持つコードと混同する日本語などのバイナリや特殊記号を16進形式にして、先頭に%を付加した文字に変換する。正確に言うと、非予約文字、予約文字以外を変換する。 項目 説明 非予約文字 ASCIIのコード範囲内の英字（大文字・小文字）、数字、ハイフン “-”、ピリオド “.”、アンダースコア “_”、チルダ “~” 予約文字 URIを表す符号。ASCIIのコード範囲内のコロン “ ”、スラッシュ “/”、疑問符 “?”、シャープ “#”、角括弧 “[”、“]”、単価記号 “@”、感嘆符 “!”、ドル記号・ペソ記号 “$”、アンパサンド “ ”、アポストロフィー・シングルクオート “ ”、丸括弧 “(”、“)”、アスタリスク “*”、正符号・加算記号 “+”、コンマ “,”、セミコロン “;”、等号 “=” name=%E8%8F%85%E7%9B%B4%E4%BA%BA UTF-8で、%E8%8F%85：菅, %E7%9B%B4 直, %E4%BA%BA 人 x-www-form-urlencoded(URLエンコード) POSTのフォームデータをエンコードする。%を付加する方式は、パーセントエンコーディングと同じ。パーセントエンコーディングの予約文字も変換している スペースは"+"に変換。Javaのソースを見ると、英数字、"-","_", ".","*"以外を変換する BASE64 Wikipediaより データを64種類の印字可能な英数字のみを用いて、それ以外の文字を扱うことの出来ない通信環境にてマルチバイト文字やバイナリデータを扱うためのエンコード方式 7ビットのデータしか扱うことの出来ない電子メールにて広く利用されている A–Z, a–z, 0–9 までの62文字と、記号2つ (+ , /) 、さらにパディング（余った部分を詰める）のための記号として = が用いられる 76文字ごとに改行コードが入る Cookie Wikipediaより クッキーを設定する際、どの要求に対してクッキー情報を送り返すのか、URLの範囲を指定する。 規定値は、クッキーを設定したサーバに対するすべての要求であり、対象を広げることも狭めることもできる。ただし広げる場合でも、トップレベルドメインより狭い範囲でなければならない。 またクッキーの有効期限は、通常はブラウザを終了するまでだが、指定した期限まではブラウザを再度起動しても保持されるように設定することができる。有効期限の情報も、サーバからブラウザにクッキー情報を送信する段階で付加される。 無期限という設定は出来ない。 詳細： http //www.studyinghttp.net/cookies リクエスト 全てテキスト。行(CRLF)で区切られる。 フォーマット リクエストラインは１行。ヘッダは複数行。 大項目 小項目 説明 リクエストライン メソッド GET,POST,PUTなどのどれか。 リクエストURL リクエストを処理するURI プロトコルバージョン HTTPのバージョン。【例】HTTP/1.1 ヘッダ HOST リクエスト先のホスト名。必須項目 空行 POSTのときにヘッダの終了を示すために挿入される メッセージボディ POSTのときにリクエストデータを出力する 【例】 GET http //example.jp/31/31-001.php HTTP/1.1 リクエストライン Host example.jp --+ User-Agent Mozilla/5.0 (Windows NT 5.1; rv 2.0.1) Gecko/20100101 Firefox/4.0.1 | Accept text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8 +-- ヘッダ Accept-Language ja,en-us;q=0.7,en;q=0.3 | Accept-Encoding gzip, deflate | Accept-Charset Shift_JIS,utf-8;q=0.7,*;q=0.7 | Keep-Alive 115 | Connection keep-alive | Referer http //example.jp/31/ --+ Accept-CharsetでShift-JISの品質係数が１であるため、サーバは、Shift-JISでエンコードしたメッセージを返す。 POST http //example.jp/31/31-003.php HTTP/1.1 リクエストライン Accept image/gif, image/jpeg, image/pjpeg, image/pjpeg, application/vnd.ms-excel ヘッダ Referer http //example.jp/31/31-002.php Accept-Language ja User-Agent Mozilla/4.0 (compatible; MSIE 8.0; Windows NT 5.1; Trident/4.0; .NET CLR 1.1.4322; .NET CLR 2.0.50727; Inf oPath.1; .NET CLR 3.0.4506.2152; .NET CLR 3.5.30729) Content-Type application/x-www-form-urlencoded Accept-Encoding gzip, deflate Host example.jp Content-Length 65 Connection Keep-Alive Pragma no-cache name=%E8%8F%85%E7%9B%B4%E4%BA%BA mail=kan@aaa.jp gender=%E7%94%B7 メッセージボディ Hostヘッダ HTTP1.1では必須。POST/GETでのURIにHOSTを指定していなかった場合、Virtualホストを特定できない。そのためHostヘッダにHOST名を指定する。URIにホスト名があって、Hostヘッダにもホスト名がある場合はURIを優先する。URIにホスト名を指定することを強制すれば良いような気がするが。 Referer ヘッダの一種。リンク元のURLを示す。あるWebページのリンクをクリックして別のページに移動したときの、リンク元のページのこと。 改変、削除される場合があるので正しくリンクを示しているとは限らない。 セキュリティ上、問題となるのは、URLが秘密情報を含んでいる場合。たとえば、URLがセッションIDを含んでいる場合。なりすましに使われる可能性がある。 【例】 GET http //example.jp/31/31-002.php HTTP/1.1 Accept image/gif, image/jpeg, image/pjpeg, image/pjpeg, application/vnd.ms-excel, application/vnd.ms-powerpoint, appl ication/msword, application/x-shockwave-flash, application/x-ms-application, application/x-ms-xbap, application/vnd.ms -xpsdocument, application/xaml+xml, */* Referer http //example.jp/31/ Accept-Language ja User-Agent Mozilla/4.0 (compatible; MSIE 8.0; Windows NT 5.1; Trident/4.0; .NET CLR 1.1.4322; .NET CLR 2.0.50727; Inf oPath.1; .NET CLR 3.0.4506.2152; .NET CLR 3.5.30729) Accept-Encoding gzip, deflate Host example.jp Connection Keep-Alive http //example.jp/31/のHTMLのaタグからリクエストが発生したため、Refererヘッダがつく。 レスポンス 全てテキスト。行で区切られる。 フォーマット ステータスラインは１行。ヘッダ、ボディは複数行。 大項目 小項目 説明 ステータスライン プロトコルバージョン ステータスコード 1XX 処理継続、2XX 正常終了、3XX リダイレクト、4XX クライアントエラー、5XX サーバエラー テキストフレーズ レスポンスヘッダ Content-Length ボディのバイト数 Content-Type MIMEタイプ; charset=エンコーディング 空行 ヘッダの終わりを示す ボディ 【例】 HTTP/1.1 200 OK Date Wed, 04 May 2011 04 51 51 GMT Server Apache/2.2.14 (Ubuntu) X-Powered-By PHP/5.3.2-1ubuntu4.2 Vary Accept-Encoding Content-Length 20 Keep-Alive timeout=15, max=100 Connection Keep-Alive Content-Type text/html; charset=UTF-8 body 13 51 /body Content-type メディアタイプを示すエンティティヘッダ（メッセージボディに対する付随情報）。type "/" subtype *( ";" parameter )であらわされる。text/html; charset=utf-8など。charsetを追加すると出力の文字コードを示す。HTML文書にapplication/octet-streamを付けると、 多くの場合、Webブラウザはそれをダウンロードするか否か問い合わせるようになる。 Apacheの場合は、httpd.confの以下の設定でcharsetを指定する。 AddDefaultCharset UTF-8 Tomcatでは、JSPのpageディレクティブで設定する。 %@ page language="java" contentType="text/html; charset=Windows-31J" pageEncoding="Windows-31J" % http //www.atmarkit.co.jp/fjava/rensai3/mojibake01/mojibake01.html 参照
https://w.atwiki.jp/atachi/pages/55.html
主なクラス [#n83d18cd] Webのリクエスト(WebClient版) [#vc4783ac] Webのリクエスト(HttpWebRequest) [#oceb8eb7] URLのエンコード [#dc0c93ce] .NET Frameworks Compactでのエンコード [#ydcce460] 主なクラス WebClient(MSDN) シンプルなWebアクセスを行う。ファイルのアップロードなどの機能を簡単に提供する。 HttpWebRequest(MSDN) HTTPプロコトルに特化したWebRequest HttpWebResponse(MSDN) HttpUtility(MSDN) HTTPに関するユーティリティクラスとして、System.Web名前空間に属するクラスがある。この名前空間はデフォルトでは読み込まれないので、プロジェクトの参照設定から手動で組み込む必要がある。.NETをコンパクトで実装しているプロジェクトの場合はこちら Webのリクエスト(WebClient版) WWWサーバー上のファイルを読み込むにはWebClient.OpenRead()を使うとHTTPを使用したリクエストをすべて作成してアクセスするので非常に簡単に該当のデータを読み込むことができる。 WebClient myClient = new WebClient(); Stream response = myClient.OpenRead("http //www.contoso.com/index.htm"); response.Close(); WebClientを使ったリクエストは非常にシンプルなリクエストしか行うことができない。 Webのリクエスト(HttpWebRequest) GETの場合はリクエストするURIにクエリー文字列を直接記述できる。 HttpWebRequest req = (HttpWebRequest)WebRequest.Create("http //hogehoge.com?mode=test"); WebResponse res = req.GetResponse(); Stream stream = res.GetResponseStream(); // ストリームからデータの読み込み stream.Close(); POSTの場合は、WebRequestから書き込み用のStreamを取得しそこにクエリーに含める文字列を追加する。 HttpWebRequest req = (HttpWebRequest)WebRequest.Create("http //hogehoge.com"); req.Method = "POST"; // POSTによるアクセスモード req.ContentType = "application/x-www-form-urlencoded"; // ContentTypeの指定は必須ではないが // WWWサーバーに対して行儀のよい作法。 // 相手のWWWサーバーの設定によっては、適切でないContentTypeは // リクエストを蹴られる場合があるので。 using(StreamWriter requestWriter = new StreamWriter(req.GetRequestStream())) { // C#基本構文(リソース)を参照 requestWriter.Write("mode=test"); } WebResponse res = req.GetResponse(); Stream stream = res.GetResponseStream(); // ストリームからデータの読み込み stream.Close(); 参考 using構文について URLのエンコード URLには日本語や一部の文字を含めることができません。 HTTPのリクエストでGETを使用する場合は必ずURLのエンコードが必要です。 たとえば、URLにはスペースを含めることができないので、URLのエンコードによって「%2F」というメタデータに変換されます。 URLのエンコードはHttpUtilityクラスのユーティリティメソッドを使います。 string encoded = HttpUtility.UrlEncode(target); .NET Frameworks Compactでのエンコード .NET Framework Compact で開発している場合、System.Webを参照できません。 代わりにUriクラスを使用してエンコードすることができます。(UTF-8のみ) string escaped = Uri.EscapeUriString("ほげほげ");